Direct measurement of 2DG6P without the use of radioisotope.Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach with which to estimate glucose uptake and thereby to explore the regulation of glucose metabolism and mechanism of insulin resistance. Radioisotope-labeled 2DG is usually used for the measurement of 2DG uptake both _III_in vivo_IIII_ and _III_in vitro_IIII_. However the radioisotope (RI) method is required a specialized facility for RI in strict limitation and cannot be handled in ordinal laboratories. Furthermore, radioactive 2DG administered into cultured cells remains in the extracellular space, and therefore the results must be corrected by separating the extracellular 2DG and intracellular 2DG/2DG-6-phosphate (2DG6P) in the cells.Thiskit is based on theenzymatic method for the direct measurement of 2DG6P amount without any use of radioisotope materials (Saito K and Minokoshi Y, _III_et al_IIII_. Analytical Biochem 412: 9-17, 2011). Advantages: 1. No RI materials are required, and 2DG uptake can be measured in any ordinal laboratories.2. Direct measurement of 2DG6P amount accumulated in target cells.3. High sensitivity with the use of enzyme-recycling amplification reaction.