Trolox is a water soluble analog of tocopherol (Vitamin E) which is a very strong antioxidant, commonly used to measure antioxidant capacity. Vitamin E is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables

RECOMMENDED USES

To compare thee-BQC results(µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples.

COMPATIBLE BUFFERS



Product	Reference
150 mM Sterile PBS pH 7.4	BQC-ST08006

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

	Standard (µl)	Diluent (µl)	TEAC (µM)
1	0	1000	0
2	50	950	50
3	100	900	100
4	200	800	200
5	400	600	400
6	600	400	600
7	800	200	800
8	1000	0	1000

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

TROLOX is more predominantly present in Q1 where it has lower detection limits and less interferences. It is recommended to use Q1 for calibrations at low concentrations of TROLOX.

	Q1	Q2	Qt
Sensitivity	0.05 µC/ µM	0.05 µC/ µM	0.11 µC/ µM
LOD	2 µM	90 µM	42 µM
LOQ	7 µM	300 µM	140 µM
LOB	0.8 µM	7 µM	8 µM
RSD	15 %	13 %	14 %

COMPARISON WITH TAC ASSAY KITS

If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an TROLOX standard could be used:

Product	Reference
DMPD	BQC-KF01001
FRAP	BQC-KF01003
FAST FRAP	BQC-KF01006
ORAC	BQC-KF01004