Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples.

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples.

COMPATIBLE BUFFERS



Reference	Product	Description
BQC-ST08006	Bio-Buffer	150 mM Sterile PBS pH 7.4
BQC-ST08007	General Buffer	100 Mm PBS pH 7
BQC-ST08005	Nutrition Buffer	0.1 M PBS pH 5.8

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

	Standard (µl)	Diluent (µl)	GAE (µM)
1	0	1000	0
2	10	990	10
3	25	975	25
4	50	950	50
5	75	925	75
6	100	900	100

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

Gallic acid is more predominantly present in Q2 where it has higher sensitivity. It is recommended to use Q2 for calibrations at low concentrations of Gallic acid.

	Q1	Q2	Qt
Sensitivity	0.02 µC/ µM	0.05 µC/ µM	0.8 µC/ µM
LOD	5 µM	10 µM	7 µM
LOQ	17 µM	30 µM	25 µM
LOB	2 µM	12 µM	14 µM
RSD	15 %	10 %	14 %