Hematoxylin-Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Mayer’s Hematoxylin does not contain mercury.Uses and sample preparation:Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.ComponentsMayer’s Hematoxylin ReagentEosin ReagentShort procedureAdd Mayer’s HematoxylinWash with warm tap waterAdd Glacial acetic acidWash with tap waterAdd Eosin solutionWash with tap waterDehydrate with graded ethanol, clear and coverslipStaining characteristics:Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple.