Cosmo/Thiol Quantification Assay Kit (-SH/S-S)/100 Tests/BQC-KB03007-100
商品编号：
BQC-KB03007-100
市场价：
¥5720.00
美元价：
3432.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The kit is designed to measure free thiols and disulfides in biological samples. Background: Bioquochem Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. Since many proteins contain redox-sensitive free thiols, the identification and quantification of their different redox states gives us an idea of the oxidative stress level of the sample. The assay described here measures the formation of 2-nitro-5-thiobenzoate (TNB), which is proportional to the amount of reduced thiols in the sample that are oxidized by the 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) through a non-specific reaction. The generated TNB ionizes to a dianion at alkaline pH and thus develops an intense yellow color with an absorbance maximum at 412 nm. On the other hand, the assay also allows the measurement of disulfides in the sample, thanks to NaBH4, which reduces these to thiols.Custom Assay Service Available! Please contact us for more information.
品牌介绍
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