For real time PCRTHUNDERBIRD® qPCR® Mix is a highly effective master mix (2 x concentration) for realtime PCR which has been developped with Taq DNA polymerase as a base.THUNDERBIRD®Probe qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan® probes. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments. This product is an improved version of Realtime PCR Master Mix (Code No. TYB-QPK-101). In particular, reaction specificity and PCR efficiency is enhanced. The TaqMan® probe system utilizes fluorescence emission from the probes. The probes hybridize to the target amplicons and then emit fluorescence upon degradation by the 5"-3" exonuclease activity of Taq DNA polymerase. This type of detection system can achieve higher specificity inreal-time PCR assays than the SYBRR Green I detection system. Features: -High specificityThe specificity for the detection of low-copy targets is improved.-Homogeneous amplificationThe dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)-Broad dynamic rangeHigh specificity and effective amplification enable the detection of a broad dynamic range.-Compatibility for various real-time cyclers.The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.-Hot start PCRThe master mix contains anti-Taq DNA polymerase antibodies for hot start technology.The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.