4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:抗体、ELISA试剂盒、细胞融合、转染、培养
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > Cosmo/GIP (Total Human) ELISA/1 Kit/YII-YK253-EX
商品详细Cosmo/GIP (Total Human) ELISA/1 Kit/YII-YK253-EX
Cosmo/GIP (Total Human) ELISA/1 Kit/YII-YK253-EX
Cosmo/GIP (Total Human) ELISA/1 Kit/YII-YK253-EX
商品编号: YII-YK253-EX
品牌: Cosmo Bio Co. Ltd
市场价: ¥16300.00
美元价: 9780.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagons-like peptide-1 (GLP-1), are a group of gastrointestinal hormones that cause an increase in the amount ofinsulin released from the beta cells ofthe islets of Langerhans after ingestion of food.The intestinal peptide GIP was first isolated from porcine upper small intestine1). The sequences ofporcine, bovineand human GIPhave been determined, each has 42 amino acids, and thesequencesis highly conserved. The porcine and bovine peptides differ from the human at two and three site,respectively. Takeda et al. have isolated a human cDNA encoding the GIP precursor and confirming thatGIP belongs to the vasoactive intestinal peptide (VIP)/Glucagon/secretin family.GIP is a gastrointestinal peptide hormone that is released from duodenal endocrine K cells after absorption ofglucose or fat. GIP is a potent releaser of insulin in experimental animals8) and in man 9,10) providedthat the blood glucose is above basal level. Plasma level of GIP is elevated after an oral glucose load or ameal in normal man. This increase after a meal is below normal in newly diagnosed insulindependentdiabetics).It is now being recognized that GIP receptor is also expressed in organs and cells such asduodenum, small intestine, pancreatic alpha-cell, adipocyte and osteoblast. These results demonstrateGIP may have a lot of physiological effect inaddition to their glucoregulatory effects.GIP is rapidly inactivated by the enzyme dipeptidyl peptidase- 4 (DPP- 4) to GIPwith a bloodhalf-life of only several minutes. DPP- 4 inhibitor can prolong the half-life of GIP, that expectingtreatment of incretin effect.The kit can be used for measurement of total GIP [both GIP (1-42) and GIP (3-42)] in human plasmawith high sensitivity. It will be a specifically useful tool for incretin research.This ELISA kit is used for quantitative determination of human total GIP in plasma and culture mediumsupernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, ithas no influence by other components in samples. GIP standard is highly purified synthetic product.Specificity:This ELISA kit has high specificity to human GIP(1-42) and GIP(3-42), and shows no cross reactivity toGlucagon, GLP-1(7-37), GLP-1(7-36) NH2, GLP-1(9-36) NH2and human GLP-2.Assay principle:This ELISA kit for determination of human total GIP is based on a sandwich enzyme immunoassay. Tothe wells of plate coated with highly purified mouse monoclonal antibody against human GIP, standardsorsamples are added for the 1st step immunoreaction. After the 1st step incubation and plate washing,HRP labeled antibody solution against human GIP is added as the 2nd step to form antibody - antigen -labeled antibody complex on the surface ofthe wells. After the 2nd step incubation and rinsing outexcess labeled antibody, Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine(TMB) and the concentration of human total GIP is calculated.
品牌介绍
(1)为生命科学的进步和发展做出贡献:我们很自豪能够将研究人员与他们继续进行重要工作,开发新产品,改善健康和挽救生命所需的工具联系在一起。(2)提供最高水平客户服务的承诺:当您需要致电给我们有关我们产品的问题时,您将始终与真实的人交谈。没有自动应答服务,保持时间长或部门之间的转移令人沮丧。如果您的订单有问题,我们将竭尽所能使其正确。(3)维持一支高效,流动的员工队伍:通过保持有限的人员精通多种任务,我们将尽可能保持灵活性,以满足客户不断变化的需求,同时保持通往日本总部和制造商的桥梁。